急求!!英语高手帮忙翻译下分子生物学的文章

发布网友 发布时间：2022-04-23 13:09

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热心网友 时间：2023-10-14 23:07

10 with 50-day-old, body weight (15 ± 1) kg on the 1st of male piglets Junmu cold stress test. Normal rearing temperature of 15 ~ 18 ℃. Test the free trial period and feed and water. Test is divided into 2 groups, each of the five piglets, respectively, in a 10 ~ a 4 ℃ and 4 ~ 10 ℃ cold environment stimulating. The two groups in the stress 0 h, 0.5 h, 3 h, 6 h, 12 h, 24 h, the stress in the restoration of 0.5 h, 3 h, 6h, 12 h, 24 h, when a total of 11 hours sampling points. Respective merits of each point in time in 5mL of blood, and quickly separated from lymphocytes, after adding 1 mL Trizol under a ultra-low temperature refrigerator 84 ℃ cryopreservation.
According to rats, cattle and wild boar conservative sequences of the HSP70 region primer design. Upstream primer: gCCAAgCtggAC AAggC; downstream primer: ttgAAgAAgtCCtgCAg. Trizol extraction kit with a total lymphocyte RNA, using AMV reverse transcriptase to reverse after PCR. DNA gel recovery after the link PMD18 a T vector and transformed into competent cells in the DH, and Green Preparation of plasmid DNA plasmid extraction kit, and PCR identification and restriction enzyme digestion. Sequencing results are as follows:
GCCAAGCTGGACAAGGCCCAGATCCACGACCTGGTGCTGGTGGGGGCTCGAC
GCGCATCCCCAAGGTGCAGAAGCTGCTGCAGGACTTCTTCAA
According to the sequence of amplified fluorescence probe design, probe sequence: AgAtCcAcgAcctggtgctg (Shanghai Saboia probe by synthetic biotechnology company). Fluorescent light-emitting group 5 markers FAM, 3 end-labeling fluorescence inhibition group TAMAR.
84 ℃ from the refrigerator out good bacteria have been identified, lessons 10, added to 5 mL LB medium. 37 ℃, 200 r / min, to spend the night. Green extraction of plasmid DNA Plasmid Preparation Kit. According to molecular size, will be translated into the quality of copy number, the gradient was diluted for quantitative template. 10 copies of recombinant plasmid by the concentration of 109 / μL, 108/μL, 107/μL, 106/μL, 105/μL, 104/μL diluted plasmid standards as a positive template for real-time fluorescence quantitative PCR conditions optimization, the standard curve and sample tests