pcr技术用的原料是dCTP等还是dCMP

发布网友 发布时间：2022-05-14 17:04

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热心网友 时间：2023-10-15 01:56

就胞嘧啶而言是dctp。就四个碱基的mix则为dntp（n=a,t,c,g）。因为是以此底物为原料，要加在3'-OH上需要高能建。dnmp是没有高能磷酸键的，而dntp则有两个。但最后在DNA链中的形式是dnmp。谢谢。

热心网友 时间：2023-10-15 01:57

是dNTP吧
A deoxyribonucleotide is the monomer, or single unit, of DNA, or deoxyribonucleic acid. Each deoxyribonucleotide comprises three parts: a nitrogenous base, a deoxyribose sugar, and one phosphate group.[1] The nitrogenous base is always bonded to the 1' carbon of the deoxyribose, which is distinguished from ribose by the presence of a proton on the 2' carbon rather than an -OH group. The phosphate groups bind to the 5' carbon of the sugar.

When deoxyribonucleotides polymerize to form DNA, the phosphate group from one nucleotide will bond to the 3' carbon on another nucleotide, forming a phosphodiester bond via dehydration synthesis. New nucleotides are always added to the 3' carbon of the last nucleotide, so synthesis always proceeds from 5' to 3'.
维基百科
印象里，dCTP和dCMP只是一个中间体。

热心网友 时间：2023-10-15 01:57

是dNTP
dNTP既是能量提供者，也是DNA的骨架组成。

热心网友 时间：2023-10-15 01:58

dNTP ,浓度20--200umol/L