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发布网友 发布时间：2022-05-08 19:41

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热心网友 时间：2024-01-03 14:25

In this experiment, groundnut meal for raw material neutral protease and alkali protease for combinatorial enzyme, alkali protease and neutral protease proportion of 3) protein peptide groundnut meal preparation. This experiment with the content of protein peptide groundnut meal for index, at different times, in different temperature, different PH, different enzyme bottom than the substrate concentration, different conditions of groundnut meal to affect the results of enzymatic hydrolysis. Experimental results show that the enzyme solution for 45 degrees Celsius temperature, enzymatic hydrolysis enzyme, the time than 1 1h 8%, substrate concentration, under the condition of enzymatic hydrolysis of fluid from the highest content of groundnut meal for peptide and 9.46 mg/ml) through the complex surface and the conclusion of the response. Will get molecular peptide liquid ultrafiltration less than 5,000 short peptide, after the composite column chromatography will rotate with short peptide separated, evaporation and concentration, use gel electrophoresis peptide liquid measure which micromolecule peptide, molecular weight range for 0.94-1.07 KD and 0.94-135 KD. This experiment by DPPH, lipid peroxidation and hydroxyl radicals method of three different oxidation of experiments to test groundnut meal peptide oxidation resistance, groundnut meal strong resistance to oxidation.