蛋白质表达一般的条件?

发布网友发布时间：2023-06-27 18:20

共3个回答

热心网友时间：2024-02-01 18:17

一般影响表达的条件是温度，培养基PH值（一般为7.0），IPTG浓度，转速等，常用条件37度，OD600为0.5－1.0间，IPTG浓度我常用0.1mmOL/L，也可以高到1.5mmol/L。转速需要自己摸索，不要高于200r/min。不同的载体和基因都不同。并没有完全适用的万能条件。

热心网友时间：2024-02-01 18:17

You have to include IPTG in log growth phase, that is, the OD600 is about 0.5. IPTG concentration can be 0.5 to 2 mM. Sometimes 0.5 mM is not enough, so to be safe, use 1 mM.

Inction time is dependent on what protein you are expressing. You can do a test by collecting samples at inction time 0, 1, 2, 4 hour time points, then run a SDS PAGE to see when is good for your interested protein.

Some protein expression may need lower temperature than 37C. Some use room temperature (25C), some even use lower than that. So you have to test it yourself. In most cases, 37C is OK.

热心网友时间：2024-02-01 18:18

IPTG 1/1000 培养到OD0.6后添加，37度诱导4小时