akt (pan) (c67e7) rabbit mab #4691中c67e7代表什么

发布网友发布时间：2022-04-20 09:04

共1个回答

热心网友时间：2022-07-12 05:31

如果某些蛋白质存在修饰位点，或稍有差异，pan antibody不能辨别这种差别。

Most protein families have different members which might share the common motif or sequence but have different function. Pan antibody can recognize the entire members in that family. It is always a good positive control for the specific member of one family.

For example:
1. Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. Cadherins are divided into type I and type II subgroups.
Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins.
Pan Cadherin antibody can reacts with ALL cadherins, it is a good Plasma Membrane Marker.
2. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis.
If you want to detect E-cadherin only, you should use E-cadherin antibody instead of Pan Cadherin antibody

Pan 就是不专一性的，比如蛋白质有isoform, a,b,c等. Pan 抗体会和所有的，至少多于一个识别。