酶切位点的保护碱基表

发布网友发布时间：2022-05-14 10:58

共1个回答

热心网友时间：2023-08-08 22:47

不同内切酶对识别位点以外最少保护碱基数目的要求（在本表中没有列出的酶，则通常需在识别位点两端至少加上6个保护碱基，以确保酶切反应的进行。）酶寡核苷酸序列链长切割率% 　　　　　　　　2 hr 20 hr Acc I GGTCGACC
CGGTCGACCG
CCGGTCGACCGG 8
10
12 0
0
0 0
0
0 Afl III CACATGTG
CCACATGTGG
CCCACATGTGGG 8
10
12 0
>90
>90 0
>90
>90 Asc I GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA 8
10
12 >90
>90
>90 >90
>90
>90 Ava I CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA 8
10
12 50
>90
>90 >90
>90
>90 BamH I CGGATCCG
CGGGATCCCG
CGCGGATCCGCG 8
10
12 10
>90
>90 25
>90
>90 Bgl II CAGATCTG
GAAGATCTTC
GGAAGATCTTCC 8
10
12 0
75
25 0
>90
>90 BssH II GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA 8
10
12 0
0
50 0
0
>90 BstE II GGGT(A/T)ACCC 9 0 10 BstX I AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT 22
24
27 0
25
25 0
50
>90 Cla I CATCGATG
GATCGATC
CCATCGATGG
CCCATCGATGGG 8
8
10
12 0
0
>90
50 0
0
>90
50 EcoR I GGAATTCC
CGGAATTCCG
CCGGAATTCCGG 8
10
12 >90
>90
>90 >90
>90
>90 Hae III GGGGCCCC
AGCGGCCGCT
TTGCGGCCGCAA 8
10
12 >90
>90
>90 >90
>90
>90 Hind III CAAGCTTG
CCAAGCTTGG
CCCAAGCTTGGG 8
10
12 0
0
10 0
0
75 Kpn I GGGTACCC
GGGGTACCCC
CGGGGTACCCCG 8
10
12 0
>90
>90 0
>90
>90 Mlu I GACGCGTC
CGACGCGTCG 8
10 0
25 0
50 Nco I CCCATGGG
CATGCCATGGCATG 8
14 0
50 0
75 Nde I CCATATGG
CCCATATGGG
CGCCATATGGCG
GGGTTTCATATGAAACCC
GGAATTCCATATGGAATTCC
GGGAATTCCATATGGAATTCCC 8
10
12
18
20
22 0
0
0
0
75
75 0
0
0
0
>90
>90 Nhe I GGCTAGCC
CGGCTAGCCG
CTAGCTAGCTAG 8
10
12 0
10
10 0
25
50 Not I TTGCGGCCGCAA
ATTTGCGGCCGCTTTA
AAATATGCGGCCGCTATAAA
ATAAGAATGCGGCCGCTAAACTAT
AAGGAAAAAAGCGGCCGCAAAAGGAAAA 12
16
20
24
28 0
10
10
25
25 0
10
10
90
>90 Nsi I TGCATGCATGCA
CCAATGCATTGGTTCTGCAGTT 12
22 10
>90 >90
>90 Pac I TTAATTAA
GTTAATTAAC
CCTTAATTAAGG 8
10
12 0
0
0 0
25
>90 Pme I GTTTAAAC
GGTTTAAACC
GGGTTTAAACCC
AGCTTTGTTTAAACGGCGCGCCGG 8
10
12
24 0
0
0
75 0
25
50
>90 Pst I GCTGCAGC
TGCACTGCAGTGCA
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT 8
14
22
24
26 0
10
>90
>90
0 0
10
>90
>90
0 Pvu I CCGATCGG
ATCGATCGAT
TCGCGATCGCGA 8
10
12 0
10
0 0
25
10 Sac I CGAGCTCG 8 10 10 Sac II GCCGCGGC
TCCCCGCGGGGA 8
12 0
50 0
>90 Sal I GTCGACGTCAAAAGGCCATAGCGGCCGC
GCGTCGACGTCTTGGCCATAGCGGCCGCGG
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA 28
30
32 0
10
10 0
50
75 Sca I GAGTACTC
AAAAGTACTTTT 8
12 10
75 25
75 Sma I CCCGGG
CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA 6
8
10
12 0
0
10
>90 10
10
50
>90 Spe I GACTAGTC
GGACTAGTCC
CGGACTAGTCCG
CTAGACTAGTCTAG 8
10
12
14 10
10
0
0 >90
>90
50
50 Sph I GGCATGCC
CATGCATGCATG
ACATGCATGCATGT 8
12
14 0
0
10 0
25
50 Stu I AAGGCCTT
GAAGGCCTTC
AAAAGGCCTTTT 8
10
12 >90
>90
>90 >90
>90
>90 Xba I CTCTAGAG
GCTCTAGAGC
TGCTCTAGAGCA
CTAGTCTAGACTAG 8
10
12
14 0
>90
75
75 0
>90
>90
>90 Xho I CCTCGAGG
CCCTCGAGGG
CCGCTCGAGCGG 8
10
12 0
10
10 0
25
75 Xma I CCCCGGGG
CCCCCGGGGG
CCCCCCGGGGGG
TCCCCCCGGGGGGA 8
10
12
14 0
25
50
>90 0
75
>90
>90